mankhwala

2019-nCOV IgGIgM Rapid Test Chipangizo (chosatumikira kamodzi)

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Mankhwala Mwatsatanetsatane

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Kuzindikira ndi Kudziwika Kwake: Chipangizo Choyesera cha 2019-nCOV / COVID-19 IgG / IgM Rapid chayerekezeredwa ndi kuyesa kwa RT-PCR kotsogola pogwiritsa ntchito zitsanzo zamankhwala. Zotsatira zikuwonetsa kuti Mayeso Ofulumira a 2019-nCoV / COVID-19 IgG / IgM Chipangizochi chimakhala chodziwika bwino komanso chapadera.

Kuyesa kwa IgG:

Njira RT-PCR Zotsatira Zonse
2019-nCOV IgG / IgM Rapid Test Chipangizo Zotsatira Zabwino Zoipa
Zabwino 233 2 235
Zoipa 35 287 322
Zotsatira Zonse 268 289 557

Kuzindikira Kwachibale: 233/268 = 86.94% (95% CI *: 82.35% -90.49%)

Makulidwe Achibale: 287/289 = 99.31% (95% CI *: 97.52% -99.92%)

Zowona: 520/557 = 93.36% (95% CI *: 90.96% -95.16%)

* Chidaliro Chanthawi

Kuyesa kwa IgM

Njira RT-PCR Zotsatira Zonse
2019-nCOV IgG / IgM Rapid Test Chipangizo Zotsatira Zabwino Zoipa
Zabwino 223 7 230
Zoipa 45 282 327
Zotsatira Zonse 268 289 557

Kuzindikira Kwachibale: 223/268 = 83.21% (95% CI *: 78.19% -87.48%)

Makulidwe Achibale: 282/289 = 97.58% (95% CI *: 95.07% -99.02%)

Zowona: 505/557 = 90.66% (95% CI *: 87.94% -92.95%)

* Chidaliro Chanthawi

2019-nCOV / COVID-19 IgG / IgM Rapid Test Chipangizo ndichachangu kwambiri chromatographic immunoassay pakuzindikira kwa IgG & IgM antibody wa Coronavirus Disease 2019 m'magazi onse a anthu, seramu, kapena plasma ngati chida chothandizira kupeza matenda a COVID-19 .

NTCHITO YOFUNIKA KUGWIRITSA NTCHITO: 2019-nCOV / COVID-19 IgG / IgM Rapid Test Chipangizo ndichachangu kwambiri chromatographic immunoassay yodziwitsa oteteza IgG & IgM antibody a Coronavirus Disease 2019 m'magazi onse a anthu, seramu, kapena plasma ngati chithandizo pakuzindikira kwa COVID -19 Matenda.

Chidule cha nkhaniyi: COVID-19 ndi matenda opatsirana opatsirana kwambiri. Anthu nthawi zambiri amakhala pachiwopsezo. Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda; Anthu omwe ali ndi kachilombo ka HIV angakhale kachilombo koyambitsa matenda. Kutengera ndikufufuza kwamatenda apano, nthawi yolumikizira ndi masiku 1 mpaka 14, makamaka masiku 3 mpaka 7. Waukulu mawonetseredwe monga malungo, kutopa ndi chifuwa youma. Kuchulukana kwa mphuno, mphuno yothamanga, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka munthawi zochepa.

MALANGIZO OGWIRITSA NTCHITO: Lolani chida choyesera, choyimira, choyimira, ndi / kapena zowongolera kuti zifike kutentha (15-30 ° C) musanayesedwe. 1. Bweretsani thumba kutenthedwe musanatsegule. Chotsani chida choyesera m'thumba losindikizidwa ndikuchigwiritsa ntchito posachedwa. 2. Ikani chida choyesera pamalo oyera komanso osalala. ? Kwa Serum kapena Plasma specimens: Pogwiritsa ntchito 10μL yotayika pipette, jambulani chithunzicho mpaka pa Fill Line, ndikusamutsa 10μL serum / plasma kupita ku chitsime cha chida choyesera, kenako onjezerani madontho awiri a buffer ndikuyamba nthawi. ? Za Magazi Athu Onse (Venipuncture / Fingerstick) Zitsanzo: Pogwiritsa ntchito 10μL yotayika pipette, ndikusamutsa dontho limodzi la magazi (pafupifupi 20μL) kupita ku chitsime cha chipangizocho, kenako onjezerani madontho awiri a buffer ndikuyamba nthawi. Chidziwitso: Zitsanzo zingagwiritsidwe ntchito pogwiritsa ntchito micropipette. 3. Yembekezani mzere wachikuda kuti uwoneke.Werengani zotsatira pa mphindi 10. Osatanthauzira zotsatirazi pakadutsa mphindi 15.

sdv

MALANGIZO: Chikwama ichi chimagwiritsa ntchito immunochromatography. Khadi loyeserera lili ndi: 1) colloidal golidi wokhala ndi cholembedwetsa cholembera cha coronavirus antigen ndikuwongolera kwapamwamba kwa zolembera za golide; 2) mizere iwiri yozindikira (mizere ya IgG ndi IgM) ndi mzere umodzi wowongolera (C mzere) wa nembanemba wa nitrocellulose. Mzere wa M umasunthika ndi anti-human anti-IgM antioclonal kuti mupeze anti-coronavirus IgM antibody; mzere wa IgG sungayende bwino ndi reagent yopeza anti-coronavirus IgG antibody; ndipo chingwe cha C sichitha ndi anti antibody. Miyezo yoyeserera ikawonjezeka pamphako la khadi loyeserera, chitsanzocho chidzapitilira limodzi ndi khadi loyeserera pogwiritsa ntchito capillary.Ngati chitsanzocho chili ndi anti-IgM, antibody amadziphatika ku colloidal cholembedwa ndi golide cholembedwa anti coronavirus antigen. Ma chitetezo a mthupi adzajambulidwa ndi anti-human IgM antibody yolemetsa pamimbayo kuti ipange mzere wofiirira wa M, kuwonetsa kuti buku la coronavirus IgM antibody ndilabwino. Ngati nyembayo ili ndi anti-IgG, antibodyyo amadziphatika ku cholembera cholembedwa ndi golide cholembedwa ndi coronavirus antigen, ndipo malo achitetezo amthupi adzajambulidwa ndi reagent yopanda mphamvu pakakhungu kuti apange mzere wofiirira wa IgG, kuwonetsa kuti buku la coronavirus Woteteza ku IgG ndiwotheka. Ngati mayeso a IgG ndi IgM alibe utoto, zotsatira zoyipa zimawonetsedwa. Khadi loyeseralo lilinso ndi mzere wowongolera zabwino C. Fuchsia control control line C iyenera kuwoneka mosasamala kanthu kuti mzere woyeserera ukuwoneka. mtundu wamagulu azida zoteteza ku chitetezo cha mthupi. Ngati chingwe chowongolera khalidwe C sichikuwoneka, zotsatira zake sizikhala zosavomerezeka, ndipo chitsanzocho chiyenera kuyesedwanso ndi khadi lina loyesa.


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