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Novel Coronavirus Neutralizing Antibody Rapid Test Chipangizo

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Mankhwala Mwatsatanetsatane

FAQ

Zogulitsa

——Kuyesa koyenera kwa Matenda a Coronavirus 2019 (2019-nCOV kapena COVID-19)

kulepheretsa antibody mu seramu / plasma / magazi athunthu. Kwa akatswiri mu Vitro Diagnostic Use Only.

Chidule

Coronaviruses yatsopano ndi ya βgenus.COVID-19 ndi matenda opatsirana opatsirana kwambiri. Anthu nthawi zambiri amakhala pachiwopsezo. Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda; Anthu omwe ali ndi kachilombo ka HIV angakhale kachilombo koyambitsa matenda. Kutengera ndikufufuza kwamatenda apano, nthawi yolumikizira ndi masiku 1 mpaka 14, makamaka masiku 3 mpaka 7. Akuluakulu mawonetseredwe monga malungo, kutopa ndi chifuwa youma. Kuchulukana kwa mphuno, mphuno yothamanga, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka munthawi zochepa.

Matenda owopsa a kupuma kwamatenda a coronavirus 2 (SARS-CoV-2, kapena 2019-nCoV) ndi kachilombo koyambitsa matenda a RNA. Ndicho chifukwa cha matenda a coronavirus 2019 (COVID-19), omwe amapatsirana mwa anthu.

SARS-CoV-2 ili ndi zomanga thupi zingapo kuphatikiza spike (S), envelopu (E), membrane (M) ndi nucleocapsid (N). Mapuloteni a spike (S) ali ndi cholandilira cholandirira (RBD), chomwe chimayang'anira kuzindikira kwa cell cell receptor, angiotensin yosintha enzyme-2 (ACE2).

Zimapezeka kuti RBD ya protein ya SARS-CoV-2 S imagwirizana kwambiri ndi receptor yaumunthu ya ACE2 yomwe imayambitsa endocytosis m'maselo am'mapapo akuya komanso kubwereza kwa ma virus.kutenga kachilombo ka SARS-CoV-2 kumayambitsa kuyankha kwa chitetezo chamthupi, zomwe zimaphatikizapo kupanga ma antibodies m'magazi. Ma antibodies obisika amateteza kumatenda amtsogolo ku ma virus, chifukwa amakhalabe m'magazi kwa miyezi mpaka zaka pambuyo pa matenda ndipo amamanga mwachangu komanso mwamphamvu kwa tizilomboti kuti tiletse kulowa kwama cell ndi kubwereza.

MFUNDO

Izi zida amagwiritsa immunochromatography. Khadi loyeserera lili ndi: 1) colloidal yokhala ndi zolemba zophatikizika zagolide zophatikizika ndi coronavirus S-RBD antigen, amatanthauza kuwongolera ndi kuwongolera mayendedwe a golide a antibody; 2) mzere wodziwika (T mzere), mzere umodzi wolozera (R mzere) ndi mzere umodzi Woyang'anira khalidwe (Cline) wa nitrocellulose nembanemba. Mzere wa T umakhala wolimba ndi puloteni ya Human ACE2 pozindikira anti-coronavirus yomwe imalepheretsa antibody; mzere wa R ndi wopanda mphamvu ndi chitetezo cha antibody; ndipo chingwe cha C sichitha kuyenda ndi anti antibody.Pamene kuchuluka koyeserera kumawonjezeredwa mu dzenje la khadi loyeserera, chitsanzocho chidzasunthira limodzi ndi khadi loyeserera motsogoleredwa ndi capillary. Ngati chitsanzocho chili ndi Novel coronavirus neutralizing antibody, antibodyyo amadziphatika ku cholelo cholembedwa ndi golide cholembedwa ndi coronavirus antigen. Mitundu yotsala ya golide yotchedwa coronavirus antigen yomwe ili ndi chitetezo cha m'thupi idzajambulidwa ndi protein ya anthu ya ACE2 yopumira pa nembanemba kuti ipange mzere wofiirira wofiira T, mphamvu ya t mzere Mofananamo ndi kuchuluka kwa antibody.

Khadi loyeseranso lili ndi mzere wolamulira bwino C ndi mzere wolozera R. Fuchsia control control line C ndi mzere wowongolera R ziyenera kuwoneka mosasamala kanthu kuti mzere woyeserera ukuwoneka Ngati mzere wowongolera C suwonekere, zotsatira zake ndi yosavomerezeka, ndipo chitsanzocho chiyenera kuyesedwanso ndi khadi lina loyeserera.

Mayendedwe oti mugwiritse ntchito

Lolani chipangizocho, choyesa, chosungira, ndi / kapena zowongolera kuti zifike kutentha (15-30 ° C) musanayesedwe.

1. Bweretsani chikwama m'chipinda chotentha musanatsegule. Chotsani chida choyesera kuchokera m'thumba losindikizidwa ndikuchigwiritsa ntchito mwachangu.

2. Ikani chida choyesera pamalo oyera komanso osalala.Kwa Seramu kapena Masampuli a Plasma: Pogwiritsa ntchito mapaipi otayika a 25µL, ndikusamutsira madontho awiri a seramu / plasma kupita ku chitsime cha chipangizocho, kenako onjezerani dontho limodzi, ndi kuyamba powerengetsera nthawi. • Pazitsanzo za Magazi Athu Onse (Venipuncture / Fingerstick): Pogwiritsa ntchito 25µL yotayika pipette, ndikusamutsira madontho atatu amwazi wathunthu (pafupifupi 75µL) pachitsime cha chipangizocho, kenaka onjezerani 1 dontho la choyimitsira, ndikuyambitsa powerengetsera nthawi.

Chidziwitso: Zitsanzo zingagwiritsidwe ntchito pogwiritsa ntchito micropipette.

3. Yembekezani mzere wachikuda kuti uwoneke.Werengani zotsatira pa mphindi 10. Musamasulire zotsatirazi pakadutsa mphindi 15.

nn

KUMASULIRA ZOTSATIRA

Otsika POSITIVE: * Mzere wachikuda m'chigawo chowongolera (C) ndi dera lazowoneraRimawoneka ndipo mzere wachikuda ukuwonekera mdera loyesaT. ndipo mphamvu ya mzere wa T ndiyochepa kuposa C mzere koma wamphamvu kuposa R mzereZotsatira zake ndizotsika pang'ono ndipo ma Neutralizing antibodies amapereka chitetezo chofooka.

Middle POSITIVE: * Mzere wachikuda m'chigawo chowongolera (C) ndi dera lolozeraRimawoneka ndipo mzere wachikuda ukuwonekera mdera loyesaT. ndipo mphamvu ya mzere wa T ndiyochepa kuposa R mzereZotsatira zake ndizopakati pomwe ma antibodies a Neutralizing amapereka chitetezo chapakati.

Mkulu ZABWINO: * Mzere wachikuda m'chigawo chowongolera (C) ndi dera lofotokozeraRimawoneka ndipo palibe mzere wachikuda womwe umawoneka mdera loyesaTZotsatira zake ndizabwino ndipo Ma antibodies osalowererapo amateteza kwambiri.

ZOYENERA: Mzere wachikuda m'chigawo chowongolera (C) ndi dera lofotokozeraRimawonekera ndipo mzere wachikuda ukuwonekera mdera loyesaT. ndipo mphamvu ya mzere wa T ndiyolimba kuposa CmzereZotsatira zake ndizosavomerezeka ndipo mulibe ma Neutralizing antibodies mthupi lanuumatengeka mosavuta ndi buku la coronavirus Ngati muli ndi kachilomboka,.

INVALID: Palibe mzere womwe udawonekera m'chigawo cha C.

Vuto losakwanira la buffer kapena njira zolakwika zosakwanira ndiye zifukwa zazikulu zolepheretsa mzere kuwunika ndikuwunika njirayo ndikubwereza ndondomekoyi ndi chida choyesera chatsopano. Ngati vutolo likupitilira, siyani kugwiritsa ntchito zida zoyeserera mwachangu ndikulumikizana ndi omwe amagawa nawo pafupi.

ZOYENERA KUCHITIKA PATSOPANO PATSOPANO

Zotsatira Zakuyembekezereka nthawi ya

katemera

Novel coronavirus yolepheretsa kachilombo koyambitsa matenda kachilombo ka HIV

Zabwino

Pakati Pabwino

Mkulu Zabwino

Zoipa

Masiku Asanachitike Mlingo 1

 

 

 

Masiku 21 pambuyo pa Mlingo 1

 

 

Masiku 7 pambuyo pa Mlingo 2

 

 

Masiku 14 pambuyo pa Mlingo 2

 

 

 

Novel Coronavirus Neutralizing Antibody Rapid Test Chipangizo

——Kuyesa koyenera kwa Matenda a Coronavirus 2019 (2019-nCOV kapena COVID-19)

kulepheretsa antibody mu seramu / plasma / magazi athunthu. Kwa akatswiri mu Vitro Diagnostic Use Only.

Chidule

Coronaviruses yatsopano ndi ya βgenus.COVID-19 ndi matenda opatsirana opatsirana kwambiri

matenda. Anthu nthawi zambiri amakhala pachiwopsezo. Pakadali pano, odwala omwe ali ndi kachilomboka

coronavirus ndiye gwero lalikulu la matenda; anthu omwe ali ndi kachilombo ka HIV angakhalenso

gwero lopatsirana. Kutengera kafukufuku wapano wamatenda, nthawi yophatikizira ndi 1

mpaka masiku 14, makamaka masiku atatu mpaka 7. Akuluakulu mawonetseredwe monga malungo, kutopa ndi chifuwa youma.

Kuchulukana kwa mphuno, mphuno yothamanga, zilonda zapakhosi, myalgia ndi kutsekula m'mimba zimapezeka munthawi zochepa.

Matenda oopsa a kupuma a coronavirus 2 (SARS-CoV-2, kapena 2019-nCoV) ndi

Anaphimba kachilombo ka RNA kosagawanika. Ndi chifukwa cha matenda a coronavirus

2019 (COVID-19), yomwe imafalikira mwa anthu.

SARS-CoV-2 ili ndi zomanga thupi zingapo kuphatikiza spike (S), envelopu (E), membrane (M)

ndi nucleocapsid (N). Prike protein (S) ili ndi cholandilira cholandirira (RBD), chomwe ndi

yofunika kuzindikira cell cell receptor, angiotensin yosintha enzyme-2 (ACE2).

Zimapezeka kuti RBD ya protein ya SARS-CoV-2 S imagwirizana kwambiri ndi ACE2 yaumunthu

cholandilira chotsogolera ku endocytosis m'maselo am'mapapo akuya komanso kubwereza kwa ma virus.

Kutengera ndi SARS-CoV-2 kumayambitsa kuyankha kwamatenda, komwe kumaphatikizapo kupanga

ma antibodies m'magazi. Ma antibodies obisika amateteza ku matenda amtsogolo

kuchokera kumavairasi, chifukwa amakhalabe mumayendedwe a miyezi kwa zaka mpaka matenda atadutsa

ndipo idzamangirira mwachangu komanso mwamphamvu ku tizilombo toyambitsa matenda kuti tipewe kulowa kwama cell ndikubwereza.

Ma antibodies amenewa amatchedwa ma antibodies osasokoneza.

MFUNDO

Izi zida amagwiritsa immunochromatography. Khadi loyeserera lili ndi: 1) yolembedwa ndi golide wokhala ndi colloidal

zophatikizanso buku la coronavirus S-RBD antigen, onaninso kuwongolera ndi kuwongolera magwiridwe anthawi zonse agolide

zolembera; 2) mzere wodziwika (T mzere), mzere umodzi wolozera (R mzere) ndi mzere umodzi wowongolera (C

Mzere) wa nitrocellulose membrane. Mzere wa T umakhala wolimba ndi puloteni ya Human ACE2 ya

kupeza coronavirus yolepheretsa antibody; mzere wa R umasunthika poyang'ana

onetsetsani antibody; ndipo chingwe cha C sichitha ndi anti antibody.

Miyezo yoyeserera ikawonjezeka panjira ya khadi loyeserera,

sampuli ipita patsogolo limodzi ndi khadi loyeserera motsogoleredwa ndi capillary. Ngati chitsanzo

ili ndi Novel coronavirus yomwe imalepheretsa anti-antibody, kuti antibody imamangiriza ku colloidal

cholembedwa ndi golide cholembedwa anti coronavirus antigen. Mabuku ena onse okhala ndi golide otchedwa coronavirus antigen mu

chitetezo cha mthupi chidzagwidwa ndi protein yaumunthu ya ACE2 yopanda mphamvu pa nembanemba kuti

pangani mtundu wofiira wa T wofiirira, mphamvu ya t mzere Mofananamo molingana ndi ndende ya

antibody.

Khadi loyesera lilinso ndi mzere wowongolera mawonekedwe C ndi mzere wofotokozera R. Mtundu wa fuchsia

mzere wolozera C ndi mzere wowongolera wowongolera R uyenera kuwoneka mosasamala kanthu kuti ndiyeso yanji

Ngati chingwe chowongolera mawonekedwe C sichikuwoneka, zotsatira zake sizowona, komanso chitsanzocho

imafunika kuyesedwanso ndi khadi lina loyeserera.

Mayendedwe oti mugwiritse ntchito

Lolani chipangizocho, choyesa, chosungira, ndi / kapena zowongolera kuti zifike kutentha (15-30 ° C) zisanachitike

kukayezetsa.

1. Bweretsani thumba ndi firiji musanatsegule.Chotsani chida choyesera kuchokera pa

thumba losindikizidwa ndikuligwiritsa ntchito posachedwa.

2. Ikani chida choyesera pamalo oyera komanso osalala.

Kwa Seramu kapena Plasma specimens:

Pogwiritsa ntchito 25µL yotayidwa pipette, ndikusamutsa madontho awiri a seramu / plasma ku

chitsime cha chida choyesera, kenako onjezerani dontho limodzi, ndikuyamba nthawi. • Za Magazi Athunthu (Venipuncture / Fingerstick) Zitsanzo:

Pogwiritsa ntchito 25µL yotayidwa pipette, ndikusamutsa madontho atatu amwazi wathunthu

(pafupifupi 75µL) pachitsanzo chabwino cha chipangizocho, kenaka onjezani dontho limodzi la buffer,

ndi kuyamba powerengetsera nthawi.

Chidziwitso: Zitsanzo zingagwiritsidwe ntchito pogwiritsa ntchito micropipette.

3. Yembekezani mzere wachikuda kuti uwoneke.Werengani zotsatira pa mphindi 10. Osatanthauzira fayilo ya

zotsatira patadutsa mphindi 15.

 

KUMASULIRA ZOTSATIRA

Otsika POSITIVE: * Mzere wachikuda m'chigawo chowongolera (C) ndi dera lazowoneraR

imawoneka ndipo mzere wachikuda ukuwonekera mdera loyesaT. ndipo mphamvu ya mzere wa T ndiyochepa

kuposa C mzere koma wamphamvu kuposa R mzereZotsatira zake ndizotsika pang'ono ndipo ma Neutralizing antibodies amapereka

chitetezo chofooka.

Middle POSITIVE: * Mzere wachikuda m'chigawo chowongolera (C) ndi dera lolozeraR

imawoneka ndipo mzere wachikuda ukuwonekera mdera loyesaT. ndipo mphamvu ya mzere wa T ndiyochepa

kuposa R mzereZotsatira zake ndizopakati pomwe ma antibodies a Neutralizing amapereka chitetezo chapakati.

Mkulu ZABWINO: * Mzere wachikuda m'chigawo chowongolera (C) ndi dera lofotokozeraR

imawoneka ndipo palibe mzere wachikuda womwe umawoneka mdera loyesaTZotsatira zake ndizabwino ndipo

Ma antibodies osalowererapo amateteza kwambiri.

ZOYENERA: Mzere wachikuda m'chigawo chowongolera (C) ndi dera lofotokozeraRimawonekera

ndipo mzere wachikuda ukuwonekera mdera loyesaT. ndipo mphamvu ya mzere wa T ndiyolimba kuposa C

mzereZotsatira zake ndizosavomerezeka ndipo mulibe ma Neutralizing antibodies mthupi lanuumatengeka mosavuta

ndi buku la coronavirus Ngati muli ndi kachilomboka,.

INVALID: Palibe mzere womwe udawonekera m'chigawo cha C.

Vuto losakwanira la buffer kapena njira zolakwika zolakwika ndizomwe zimayambitsa

kuwongolera mzere kulephera Onaninso njirayi ndikubwereza ndondomekoyi ndi chida chatsopano choyesera. Ngati fayilo ya

Vutoli likupitilira, siyani kugwiritsa ntchito zida zoyeserera nthawi yomweyo ndipo muthane ndi omwe amagawa nawo pafupi.

ZOYENERA KUCHITIKA PATSOPANO PATSOPANO

Zotsatira Zakuyembekezereka nthawi ya

katemera

Novel coronavirus yolepheretsa kachilombo koyambitsa matenda kachilombo ka HIV

Zabwino

Pakati Pabwino

Mkulu Zabwino

Zoipa

Masiku Asanachitike Mlingo 1

 

 

 

Masiku 21 pambuyo pa Mlingo 1

 

 

Masiku 7 pambuyo pa Mlingo 2

 

 

Masiku 14 pambuyo pa Mlingo 2

 

 

 


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