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Novel Coronavirus (SARS-Cov-2) Antigen Rapid Test Chipangizo (nasal swab)

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Mwachidule

Ma coronaviruses achilendowo ndi a βgenus. COVID-19 ndi matenda opatsirana opatsirana kwambiri. Anthu nthawi zambiri amakhala pachiwopsezo. Pakadali pano, odwala omwe ali ndi kachilombo ka coronavirus ndiye gwero lalikulu la matenda; Anthu omwe ali ndi kachilombo ka HIV angakhale kachilombo koyambitsa matenda. Kutengera kafukufuku wapano wamatenda, nthawi yophatikizira ndi 1

mpaka masiku 14, makamaka masiku atatu mpaka 7. Mawonetseredwe akulu akuphatikizapo malungo, kutopa ndi kutsokomola kouma. Kuchulukana kwa m'mimba, mphuno, kukhosi, myalgia ndi kutsekula m'mimba zimapezeka munthawi zochepa. Matenda owopsa a kupuma - coronavirus- 2 (SARS-CoV-2) ndi kachilombo kovundikira, kosagawika ka HIV kachilombo ka RNA. Ndicho chifukwa cha matenda a Coronavirus-0 (COVID-19) ofala kwa anthu amapatsirana. SARS-CoV-2 ili ndi zomanga thupi zingapo, kuphatikiza spike (S), envelopu (E), membrane (M) ndi nucleocapsid (N).

Pakadali pano, pali mitundu ingapo ya Novel coronavirus (SARS-CoV-2), komanso kusintha kwa N501Y ndi mitundu yake pafupifupi yakopa chidwi chifukwa malo awo amasinthidwe ali mu spike glycoprotein's receptor-binding domain of the virus, potero amasintha kachilombo koyambitsa matendawa. Pakuwunika kwa silico kunawonetsa kuti kusintha kwa N501Y sikunasinthe mapuloteni oyambira komanso apamwamba a dera la RBD. Chifukwa chake, antigenicity yake sinasinthe.

MFUNDO】

Novel Coronavirus (SARS-Cov-2) Antigen Rapid Test Device (nasal swab) ndi immunochromatographic membrane testay yomwe imagwiritsa ntchito ma anti-monoclonal antibodies ku Novel coronavirus.

Mzere woyeserera umapangidwa ndi magawo atatu otsatirawa, omwe ndi sampuli, reagent pad ndi membrane membrane. Nembanemba reagent muli colloidal-golide conjugated ndi chitetezo monoclonal ndi Novel coronavirus; nembanemba yomwe imakhala ndi ma antibodies achiwiri a Novel coronavirus, ndi ma polyclonal antibodies olimbana ndi mbewa ya globulin, yomwe imayambitsidwa pakatikati.

Sampulo yamphongo ikalandiridwa ndi mayeso, yankho lolumikizidwa kuchokera pa reagent pad limasungunuka ndikusunthira limodzi ndi nyemba yammphuno. Novel coronavirus ikupezeka mchitsanzo cha mphuno, zovuta zimapangidwa pakati pa anti-Novel coronavirus conjugate ndipo kachilomboka kadzagwidwa / kudziwika ndi anti-Novel coronavirus monoclonal yokutidwa pa Tregion. Kaya chitsanzocho chili ndi kachilomboka kapena ayi, yankho likupitilizabe kusunthika kuti likakumanenso ndi reagent (anti-mbewa IgG antibody) yomwe imamanga ma conjugates otsala, potero imapanga mzere wofiira m'chigawo cha C. Novel Coronavirus (SARS-Cov-2 ) Antigen Rapid Test Device (nasal swab) imatha kudziwa zonse za SARS-Cov-2 nucleoprotein komanso protein ya SARS-Cov-2. Wolemba ELISA, tidatsimikiza kuti antibody omwe timagwiritsa ntchito amamanga amino acid 511-531 a protein ya SARS Cov-2.

Kudziwika kwa mitundu ya ma SARS-CoV-2 kunayesedwa pofufuza kukhudzika kwa mapuloteni otsekemera a SARS-Cov-2 (319 mpaka 541aa). M'mayesowa, Novel Coronavirus (SARS-Cov-2) antigen-test test idakwaniritsa zomwezo pozindikira mitundu ya B.1.1.7 (UK) ndi B.1.351 (SA) monga momwe zimapezera kusiyana kwake.

AG ZOKHUDZA】

Nembanemba reagent muli colloidal-golide conjugated ndi chitetezo monoclonal ndi Novel coronavirus; nembanemba yomwe imakhala ndi ma antibodies achiwiri a Novel coronavirus, ndi ma polyclonal antibodies olimbana ndi mbewa ya globulin, yomwe imayambitsidwa pakatikati.

Kusonkhanitsa Kwambiri ndi Kukonzekera】

1. Kutola zitsanzo:

Pendeketsani mutu wa wodwalayo kumbuyo kwa 70 degree, kwinaku mukuzungulirazungulira swab, ikani swab yochepera inchi imodzi (pafupifupi 2 cm) mu mphuno (mpaka kukana kukumana ndi turbinate) .Zungunutsani kasanu kasanu motsutsana ndi khoma lammphuno. Pogwiritsa ntchito swab yemweyo bwerezani njira yosonkhanitsira mphuno yachiwiri. Chotsani swab pang'onopang'ono.

Chenjezo: Ngati ndodo ya swab imathyoka panthawi yosonkhanitsa zitsanzo, bweretsani zosonkhanitsira ndi swab yatsopano.

2. Kukonzekera kwa zitsanzo:

1) Tulutsani botolo limodzi la Zitsanzo Zofukula Zotsitsa, chotsani botolo la botolo, onjezerani chomenyera chilichonse mu chubu.

2) Ikani swab mu chubu chojambulira chomwe chili ndi Zitsanzo Zofukula Zogulitsa. Tembenuzani theswab mkati mwa chubu pogwiritsa ntchito zozungulira mozungulira mbali yazitsulo kuti madzi amveke ndikubwezeretsanso pa swab, chotsani swab. Yankho lotulutsidwa lidzagwiritsidwa ntchito ngati mayeso.

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【Malangizo OGWIRITSA NTCHITO】

Lolani mayeso, choyimira, cholumikizira chotsitsa kuti chikhale chofanana ndi kutentha kwapakati (15-30 ° C) musanayesedwe.

1. Tsegulani kapu yonse ya chubu chosonkhanitsira, tengani botolo limodzi la Zitsanzo Zofukula Zotsitsa, chotsani kapu ya botolo, onjezerani chomenyera chilichonse mu chubu.

2. Ikani chojambulira cha swab chojambulira muzitsulo zowonjezera. Sinthirani swab kwa masekondi pafupifupi 10 kwinaku mukugwedeza mutu mkati mwa chubu kuti mutulutse antigen mu swab.

3.Chotsani swab yolera yotseketsa kwinaku mukufinya mutu wosawilitsidwa pakati pa Buffer mukamachotsa kuti mutulutse madzi ambiri momwe mungathere. Tayani swab yolera yotseketsa molingana ndi pulogalamu yanu yotaya zinyalala.

4. Pukutani ndi kumata kapuyo pa chubu chosonkhanitsira, kenako sinthani chubu chojambuliracho mwamphamvu kuti musakanize chojambuliracho ndi choyikapo chotengera. Onani fanizo 4.

5. Onjezerani madontho atatu a yankho (pafupifupi 80ul) ku chitsanzocho bwino ndikuyamba nthawi.

6. Werengani zotsatira zake pa mphindi 10 ~ 20. Osatanthauzira zotsatira pambuyo pa mphindi 20.

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【KUMASULIRA ZOTSATIRA】

ZABWINO: Kuonekera mizere iwiri yofiira. Mzere umodzi wofiira umapezeka m'dera lolamulira (C), ndi mzere umodzi wofiira mdera loyesa (T). Mthunzi wautundu umatha kusiyanasiyana, koma uyenera kuganiziridwa kuti ndiwothandiza nthawi iliyonse yomwe pamakhala mzere wakakomoka.

ZOIPA: Mzere umodzi wokha wofiira umapezeka mdera lolamulira (C), ndipo palibe mzere m'chigawo choyesera (T). Zotsatira zoyipa zikuwonetsa kuti mulibe tinthu ta Novel coronavirus muzoyeserazo kapena kuchuluka kwa ma tinthu tating'onoting'ono tatsika pang'ono.

Zosayenera: Palibe mzere wofiira womwe ukuwoneka m'dera lolamulira (C). Mayesowa ndi osavomerezeka ngakhale pali mzere pagawo loyesa (T). Mavoliyumu osakwanira kapena njira zolakwika panjira ndiye zifukwa zazikulu zolepheretsa mzere kulamulira. Onaninso njira yoyeserera ndikubwereza mayesowo pogwiritsa ntchito chida chatsopano choyesera. Ngati vutolo likupitilira, siyani kugwiritsa ntchito zida zoyeserera mwachangu ndikulumikizana ndi omwe amagawa nawo pafupi.


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